Voltammetric and liquid chromatographic identification of organic products of microwave-assisted wet ashing of biological samples

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00170a008· OSTI ID:6668704

Pratt, K W; Kingston, H M; MacCrehan, W A; Kock, W F

Residual organic species in nitric acid digest of freeze-dried bovine liver (NBS SRM 1577a) have been identified by use of voltammetry, liquid chromatography, spectrophotometry, and classical chemical tests. Data from these techniques show that major products of microwave-assisted dissolution by nitric acid include o-, m-, and p-nitrobenzoic acids (NBA). In addition to these compounds, other organic species present in these digests irreversibly complex copper, but not zinc, and result in low values for copper by polarography. The NBAs and these other organic species are all eliminated by refluxing the nitric acid digest in perchloric acid at atmospheric pressure. Polarographic results obtained for copper following treatment with perchloric acid agree with the certified value. The use of voltammetry in the evaluation of wet ashing procedures is discussed.

• Research Organization: National Bureau of Standards, Gaithersburg, MD (USA)
• OSTI ID: 6668704
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 60:19; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
BIOLOGICAL MATERIALS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
EXPERIMENTAL DATA
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
SPECTROPHOTOMETRY
VOLTAMETRY
WET ASHING