Structure of a pre-mRNA branch point/3' splice site region

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.3.704· OSTI ID:6659526

Hall, K B; Green, M R; Redfield, A G

The authors analyzed the solution structure of RNA containing the branch point/3' splice site region of the first intervening sequence (IVS1) of human ..beta..-globin pre-mRNA by nuclease mapping and NMR. Nuclease mapping indicates that there are two distinct structural domains: one contains the branch point region, and the other includes the 3' splice site and second exon sequences. In the branch point domain, the adenosine at which the RNA branch forms appears to be in the loop of a stem/loop structure. The branch point structure does not appear to interact with other parts of the RNA, since its unique nuclease digestion pattern is conserved among transcripts containing the entire intron or only the branch point region. This is confirmed by a comparison of the NMR spectra of two RNA transcripts; a distinct set of resonances appears in the spectra of the RNA containing only branch sequences or including 3' splice site/exon 2 sequences. NMR studies further show that the 3' splice site/exon 2 domain has a lower melting temperature than the branch point domain, suggesting that the two regions are distinct dynamically as well as structurally. Nuclease mapping studies of adenovirus major late IVS1 indicate that this RNA has structural features in common with the human ..beta..-globin transcript.

• Research Organization: Brandeis Univ., Waltham, MA (USA)
• OSTI ID: 6659526
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:3; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BARYONS
ELEMENTARY PARTICLES
ENZYMES
ESTERASES
FERMIONS
GLOBIN
HADRONS
HYDROLASES
MAGNETIC RESONANCE
MAMMALS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEASES
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PRIMATES
PROTEINS
PROTONS
RESONANCE
RNA
SPECTRA
VERTEBRATES