The cellular metabolism of lead and calcium: A kinetic analysis in cultured osteoclastic bone cells

Conference · Wed Jan 01 04:00:00 EST 1986

OSTI ID:6654810

Rosen, J F; Pounds, J G

Characterization of lead metabolism in bone is necessary to understand the role of skeletal lead, an endogenous source of this toxic metal, in the expression of adverse effects of lead intoxication in concert with external sources. Moreover, it has been postulated that an early manifestation of lead toxicity common to diverse cell types may be pertubations in intracellular calcium homeostasis. Furthermore, it has been suggested that calciotropic hormones may express their actions on bone cells by modulating the messenger functions of intracellular Ca. Before these postulates can be more fully tested, the homeostasis of intracellular Pb and Ca must be understood more clearly. We have designed experiments to characterize the steady-state kinetic distribution and behavior of /sup 210/Pb and /sup 45/Ca in osteoclastic bone cells and to identify biological structures or functions associated with the kinetic pools. Cultures were labeled with /sup 210/Pb or /sup 45/Ca and the kinetic parameters were obtained by analysis of isotope washout curves. Cellular metabolism was defined by three kinetic pools of intracellular Pb and Ca. The largest and slowest exchanging pool was characterized as a mitochondrial compartment for both metals. These data indicate that Pb and Ca share similar intracellular pathways in osteoclastic bone cells and that both metals are readily exchangeable in this in vitro system. The results of other experiments have revealed that lead, at relatively low medium concentrations, produces a marked increase in all three intracellular Ca compartments. 18 refs., 2 figs.

• Research Organization: Albert Einstein Coll. of Medicine, Bronx, NY (USA). Dept. of Pediatrics; Brookhaven National Lab., Upton, NY (USA)
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 6654810
• Report Number(s): BNL-39108; CONF-8611123-1; ON: DE87005388
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ALPHA DECAY RADIOISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BODY
BONE CELLS
BONE TISSUES
CALCIUM 45
CALCIUM ISOTOPES
CELL CONSTITUENTS
CELL CULTURES
CHEMICAL ANALYSIS
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
HEAVY NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEAD 210
LEAD ISOTOPES
METABOLISM
MITOCHONDRIA
NONDESTRUCTIVE ANALYSIS
NUCLEI
ORGANOIDS
RADIOISOTOPES
SOMATIC CELLS
SUBCELLULAR DISTRIBUTION
TISSUES
TRACER TECHNIQUES
X-RAY EMISSION ANALYSIS
X-RAY FLUORESCENCE ANALYSIS
YEARS LIVING RADIOISOTOPES