Comparative mapping on the mouse and human X chromosomes of a human cDNA clone encoding the vasopressin renal-type receptor (AVP2R)

Journal Article · Mon Feb 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1085· OSTI ID:6652830

Faust, C J; Gonzales, J C; Seibold, A; Birnbaumer, M; Herman, G E ^[1]

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1. Baylor College of Medicine, Houston, TX (United States)

Mutation in the gene for the human renal-type vasopressin receptor (V2R) have recently been identified in patients with nephrogenic diabetes insipidus (NDI). Both V2R and NDI have been independently mapped to Xq28. Using a combination of genetic and physical mapping, we have localized the murine V2r locus to within 100 kb of L1Cam on the mouse X chromosome in a region syntenic with human Xq28. Based on conserved gene order of mouse and human loci in this region, physical mapping using DNA derived form human lymphoblasts has established that the corresponding human loci V2R and L1CAM are linked within 210 kb. The efficiency and precision of genetic mapping of V2r and other loci in the mouse suggest that it might be easier to map additional human genes in the mouse first and infer the corresponding human location. More precise physical mapping in man could then be performed using pulsed-field gel electrophoresis and/or yeast artificial chromosomes. 16 refs., 1 fig. 1 tab.

• OSTI ID: 6652830
• Journal Information: Genomics; (United States), Vol. 15:2; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN X CHROMOSOME
GENETIC MAPPING
RECEPTORS
GENE MUTATIONS
VASOPRESSIN
DNA
EFFICIENCY
ELECTROPHORESIS
GENES
METABOLIC DISEASES
MUTATIONS
PATIENTS
CHROMOSOMES
DISEASES
HETEROCHROMOSOMES
HORMONES
HUMAN CHROMOSOMES
MAPPING
MEMBRANE PROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
X CHROMOSOME
550400* - Genetics