Nerve growth induces 5-HT sub 3 recognition sites in rat pheochromocytoma (PC12) cells
- A.H. Robins Research Laboratories, Richmond, VA (USA)
In rat pheochromocytoma (PC12) cells, nerve growth factor (7S NGF) induced the expression of recognition sites that bind the specific 5-HT{sub 3} antagonist (S-) ({sup 3}H) zacopride. Culturing PC12 cells for 8-12 days in the presence of 50 ng/ml NGF increased the density (B{sub max}) of (S-) ({sup 3}H) zacopride binding sites in cell membranes (0-100,000 x g fraction) from 0 to 105 fmoles/mg protein. This binding exhibited high affinity for (S-) ({sup 3}H) zacopride (K{sub d}=0.8 nM), was specific (>95%), and was inhibited by 5-HT{sub 3} compounds with a rank of potency (quipazine>ICS 205-930 > GR38032F > BRL 24924{approx}MDL 72222 > phenylbiguanide {le} seroton-in > 2-methyl-serotonin > metoclopramide) which was distinct from neuroblastoma cells. Thus, NGF-differentiated PC12 cells possess a 5-HT{sub 3} receptor and should be useful to investigate its regulation and biochemical mechanism of action.
- OSTI ID:
- 6650311
- Journal Information:
- Life Sciences; (USA), Vol. 46:20; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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