Salicylhydroxamic acid (SHAM) inhibition of the DIC-pump in unicellular algae

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6650250

Goyal, A; Tolbert, N E ^[1]

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1. Michigan State Univ., East Lansing (USA)

SHAM at 1 or 2 mM inhibits dissolved inorganic carbon (DIC) concentrating mechanisms in unicellular green algae as measured by photosynthetic oxygen evolution or by {sup 14}C-inorganic carbon uptake (using silicone oil centrifugation techniques). This inhibition was reversed by high levels of DIC whereby the cells do not require the concentrating mechanism. SHAM inhibited the DIC-pump, which uses external CO{sub 2}, in three species of algae, Dunaliella tertiolecta, Chlamydomonas reinhardtii, and Scenedesmus obliquus when adapted to low CO{sub 2} and assayed around neutral pH. Scenedesmus adapted to air at pH 9.0 to use external HCO{sub 3}{sup {minus}} were not affected by SHAM. It is important to establish low optimum concentrations of SHAM, which varied with the algal species. The mechanism of SHAM inhibition of the CO{sub 2} concentrating process is unknown. SHAM inhibits alternative respiration in these algae, but SHAM may also inhibit other reactions involving H{sup +} gradients or transporters associated with the DIC-pump.

• OSTI ID: 6650250
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALGAE
AMINES
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
CARBON
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON DIOXIDE FIXATION
CARBON OXIDES
CHALCOGENIDES
ELEMENTS
GENETIC VARIABILITY
HYDROXAMIC ACIDS
HYDROXY COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANE TRANSPORT
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PLANTS
RESPIRATION
TRACER TECHNIQUES