Binding sites for atrial natriuretic factor (ANF) in brain: alterations in Brattleboro rats

Journal Article · Mon Dec 01 00:00:00 EST 1986 · Brain Res. Bull.; (United States)

DOI:https://doi.org/10.1016/0361-9230(86)90088-2· OSTI ID:6647209

McCarty, R; Plunkett, L M

Binding sites for atrial natriuretic factor (ANF-28) were analyzed in discrete brain areas of Brattleboro rats with hereditary diabetes insipidus and Long-Evans (LE) controls by quantitative autoradiography. The maximum binding capacity (Bmax) and affinity constant (Ka) for /sup 125/I-ANF-28 were elevated significantly in the subfornical organ of Brattleboro rats compared to matched LE controls. In contrast, values for Bmax and Ka for /sup 125/I-ANF-28 binding in choroid plexus and area postrema were similar for rats of the two strains. These findings are consistent with a selective upregulation of ANF-28 binding sites in the subfornical organ of Brattleboro rats which exhibit a profound disturbance in body fluid homeostasis. These alterations in ANF-28 binding sites in the subfornical organ may represent a compensatory response to the absence of vasopressin in the Brattleboro rat.

• Research Organization: Univ. of Virginia, Charlottesville
• OSTI ID: 6647209
• Journal Information: Brain Res. Bull.; (United States), Vol. 6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HORMONES
RADIORECEPTOR ASSAY
AUTORADIOGRAPHY
BRAIN
CHEMICAL BONDS
IODINE 125
LABELLED COMPOUNDS
RATS
RECEPTORS
VASOPRESSIN
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
RODENTS
TRACER TECHNIQUES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques