Improved method for the isolation of biosurfactant glycolipids from Rhodococcus sp. Strain H13A
Journal Article
·
· Applied and Environmental Microbiology; (USA)
OSTI ID:6644383
- Univ. of Georgia, Athens (USA)
Rhodococcus sp. strain H13A (previously name Arthrobacter sp. strain H13A) degrades haxadecane and produces exocellular glycolipids, one or more of which are biosurfactants. An improved method for the isolation of the biosurfactant glycolipids by using XM 50 diafiltration and isopropanol precipitation was devised. This procedure was advantageous since it removes protein coisolated when the glycolipids are obtained by organic extraction and silicic acid chromatography. The protein apparently does not contribute any biosurfactant characteristics to the glycolipids. The deacylated glycolipid backbone included only a disaccharide.
- OSTI ID:
- 6644383
- Journal Information:
- Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:5; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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