Molecular light switch for DNA: Ru(bpy) sub 2 (dppz) sup 2+

Journal Article · Wed Jun 06 04:00:00 EDT 1990 · Journal of the American Chemical Society; (USA)

DOI:https://doi.org/10.1021/ja00168a052· OSTI ID:6644275

Friedman, A E; Barton, J K ^[1]; Chambron, J C; Sauvage, J P ^[2]; Turro, N J ^[3]

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1. California Institute of Technology, Pasadena (USA) Columbia Univ., New York, NY (USA)
2. Universite Louis Pasteur, Strasbourg (France)
3. Columbia Univ., New York, NY (USA)

Considerable research has focused on the development of nonradioactive probes for nucleic acids. Extensive photophysical studies indicate that Ru(phen){sub 3}{sup 2+} bound to double-helical DNA displays an increase in luminescence owing to intercalation; emission from the metal-to-ligand charge transfer (MLCT) excited state decays as a biexponential with one lifetime of 2 {mu}s attributed to the intercalative form and a second lifetime of 0.6 {mu}s (indistinguishable from the free species) assigned to the surface bound form. Here we report the application of a novel transition-metal complex as a true molecular light switch for DNA. This probe is Ru(bpy){sub 2}(dppz){sup 2+} (bpy = 2,2{prime}-bipyridine, dppz = dipyrido(3,2-a:2{prime},3{prime}-c)phenazine), which shows no photoluminescence in aqueous solution at ambient temperatures, but displays intense photoluminescence in the presence of double-helical DNA, to which the complex binds avidly.

• OSTI ID: 6644275
• Journal Information: Journal of the American Chemical Society; (USA), Journal Name: Journal of the American Chemical Society; (USA) Vol. 112:12; ISSN 0002-7863; ISSN JACSA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CHEMICAL ANALYSIS
COMPLEXES
DATA
DATA ANALYSIS
DNA
EXPERIMENTAL DATA
INFORMATION
LUMINESCENCE
MEASURING METHODS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PHOTOLUMINESCENCE
RUTHENIUM COMPLEXES
TRANSITION ELEMENT COMPLEXES