A sensitive radioisotopic method for the measurement of NAD(P)H: Its application to the assay of metabolites and enzymatic activities
Journal Article
·
· Analytical Biochemistry; (USA)
- Brussels Free Univ. (Belgium)
A radioisotopic method for the assay of NADH or NADPH is presented, which is based on the conversion of 2-(U-{sup 14}C)ketoglutarate to {sup 14}C-labeled glutamate in the reaction catalyzed by glutamate dehydrogenase. The efficiency of the method is close to 75%, its precision (coefficient of variation) close to 5%, and its sensitivity close to 0.1 pmol/sample. This simple and rapid method can be applied to the measurement of several metabolites and enzymatic activities. In the present study, its application to the assay of sorbitol, 3-hydroxybutyrate, glutamate dehydrogenase, 3-hydroxybutyrate dehydrogenase, and glyceraldehyde-3-phosphate dehydrogenase is documented.
- OSTI ID:
- 6643958
- Journal Information:
- Analytical Biochemistry; (USA), Journal Name: Analytical Biochemistry; (USA) Vol. 186:2; ISSN ANBCA; ISSN 0003-2697
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
CARBOXYLIC ACIDS
COENZYMES
ENZYME ACTIVITY
ENZYMES
GLUTAMIC ACID
ISOTOPE APPLICATIONS
MAMMALS
METABOLITES
NADH2
NADP
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
RADIOASSAY
RATS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
CARBOXYLIC ACIDS
COENZYMES
ENZYME ACTIVITY
ENZYMES
GLUTAMIC ACID
ISOTOPE APPLICATIONS
MAMMALS
METABOLITES
NADH2
NADP
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
RADIOASSAY
RATS
RODENTS
TRACER TECHNIQUES
VERTEBRATES