Synthesis and circularization of N- and B-tropic retroviral DNA in Fv-1 permissive and restrictive mouse cells

Journal Article · Thu May 01 00:00:00 EDT 1980 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.77.5.2994· OSTI ID:6643303

Yang, W K ^[1]; Kiggans, J O; Yang, D M; Ou, C Y; Tennant, R W; Brown, A; Bassin, R H

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1. Oak Ridge National Lab., TN

Production of various forms of nonintegrated viral DNA was measured in cultured mouse cells carrying different Fv-1 alleles early after infection with N-tropic or B-tropic retroviruses. Quantitative analyses were performed by agarose gel electrophoresis, transfer to diazobenzyloxymethyl-paper, and molecular hybridization. In permissive infection of Fv-1/sup n/ cells (NIH Swiss and DBA mouse strains) with N-tropic virus and of Fv-1/sup b/ cells (BALB/c and C57BL/6 strains) with B-tropic virus, form III (double-stranded linear) DNA first appeared at 3 to 4 hr and reached a maximum at 8 to 10 hr; two form I (closed circle) DNAs appeared at 7 to 8 hr and reached a maximum at or beyond 12 hr. In the two Fv-1/sup b/ cells infected with N-tropic virus and in DBA (Fv-1/sup n/) cells infected with B-tropic virus, formation of the two form I DNAs was quantitatively restricted but formation of form III DNA was unaltered. In Fv-1/sup n/ NIH Swiss mouse embryo cells infected with B-tropic virus, the level of form III DNA was markedly depressed and hence the two form I DNAs were not detectable. In C57BL/6 cells as well as in DBA/2 cells 12 hr after infection, the quantity of form III DNA varied directly with the amount of restricted virus, whereas the quantity of form I DNA varied according to the square of the amount of restricted virus. The significance of these results for understanding the molecular basis of retrovirus replication and its restriction by the Fv-1 gene is discussed.

• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6643303
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 77:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
BIOSYNTHESIS
VIRUSES
CELL CULTURES
DNA REPLICATION
INFECTIVITY
MICE
QUANTITATIVE CHEMICAL ANALYSIS
RESPONSE MODIFYING FACTORS
ANIMALS
CHEMICAL ANALYSIS
MAMMALS
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RODENTS
SYNTHESIS
VERTEBRATES
550700* - Microbiology