Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: Kinetics and interactions between substrates

Journal Article · Tue May 01 04:00:00 EDT 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6643104

Folsom, B R ^[1]; Chapman, P J; Pritchard, P H ^[2]

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1. Technical Resources, Inc., Gulf Breeze, FL (USA)
2. Environmental Protection Agency Environmental Research Laboratory, Gulf Breeze, FL (USA)

Intact cells of Pseudomonas cepacia G4 completely degraded tricloroethylene (TCE) following growth with phenol. Degradation kinetics were determined for both phenol, used to induce requisite enzymes, and TCE, the target substrate. Apparent K{sub s} and V{sub max} values for degradation of phenol by cells were 8.5 {mu}M and 466 nmol/min per mg of protein, respectively. At phenol concentrations greater than 50 {mu}M, phenol degradation was inhibited, yielding an apparent second-order inhibitory value, K{sub SI}, of 0.45 mM as modeled by the Haldane expression. A partition coefficient for TCE was determined to be 0.40 {plus minus} 0.02, (TCE{sub air})/(TCE{sub water}), consistent with Henry's Law. To eliminate experimental problems associated with TCE volatility and partitioning, a no-headspace bottle assay was developed, allowing for direct and accurate determinations of aqueous TCE concentration. By this assay procedure, apparent K{sub s} and V{sub max} values determined for TCE degradation by intact cells were 3 {mu}M and 8 nmol/min per mg of protein, respectively. Following a transient lag period, P.cepacia G4 degraded TCE at concentrations of a least 300 {mu}M with no apparent retardation in rate. Consistent with K{sub s} values determined for degradation, TCE inhibited phenol degradation.

• OSTI ID: 6643104
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:5; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DECOMPOSITION
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROXY COMPOUNDS
KINETICS
METABOLISM
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PHENOL
PHENOLS
PSEUDOMONAS