Thyrotropin dependent and independent thyroid cell lines selected from FRTL-5 derived tumors grown in nude mice

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · Endocrinology; (USA)

DOI:https://doi.org/10.1210/endo-127-1-419· OSTI ID:6640588

Ossendorp, F A; Bruning, P F; Schuuring, E M; Van Den Brink, J A; van der Heide, D; De Vijlder, J J; De Bruin, T W ^[1]

---

1. Netherlands Cancer Institute, Amsterdam (Netherlands)

FRTL-5 cells were used to set up a thyroid tumor model system in C3H nu/nu mice. FRTL-5 tumors could be grown in nude mice provided serum TSH levels were elevated. Persistent TSH elevation was obtained by administration of Na131I, rendering the mice hypothyroid. After 4 weeks FRTL-5 cells were injected sc resulting in tumor growth within 2 weeks in eight out of eight mice. Although the tumors showed an apparently undifferentiated histology, lacking normal follicular structures, they were functional since the tumors were capable of concentrating (131)iodine, as demonstrated by nuclear imaging. From one of the tumors a new cell line was isolated (FRTL-5/T) that, like the parental FRTL-5 cell line, was TSH dependent for growth. In a control group of six euthyroid nude mice FRTL-5 tumor growth could not be obtained with one exception. After 3 months one animal developed a small tumor that grew rapidly thereafter. This tumor was easily transplantable in other euthyroid nude mice, showed an undifferentiated histology, and was nonfunctional, as it could not concentrate (131)iodine. From this tumor two cell lines were derived: one cultured in the presence of TSH (FRTL-5/TP) and one in the absence of TSH (FRTL-5/TA). The cell lines were analyzed for TSH responsive functions and TSH receptor expression. Responsiveness to TSH in FRTL-5/T and the parental FRTL-5 cell line were similar for most thyroid specific functions tested. However, FRTL-5/T was less sensitive than FRTL-5 for TSH induced (3H)thymidine incorporation. Both cell lines had two classes of TSH binding sites with high and low affinity respectively. FRTL-5/TP and FRTL-5/TA were both able to grow in TSH free medium and were nonresponsive to TSH in vitro, as tested for (3H)thymidine and (3H)uridine incorporation, iodine uptake, thyroglobulin iodination, and thyroglobulin secretion.

• OSTI ID: 6640588
• Journal Information: Endocrinology; (USA), Journal Name: Endocrinology; (USA) Vol. 127:1; ISSN ENDOA; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL DIVISION
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
ENDOCRINE GLANDS
ENZYMES
EXPERIMENTAL NEOPLASMS
GENE REGULATION
GLANDS
GLOBULINS
GROWTH
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
IMAGE PROCESSING
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MESSENGER-RNA
MICE
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PEROXIDASES
PITUITARY HORMONES
PROCESSING
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RATS
RECEPTORS
RIBOSIDES
RNA
RODENTS
THYMIDINE
THYROGLOBULIN
THYROID
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TSH
TUMOR CELLS
URACILS
URIDINE
VERTEBRATES