Evaluation of monoclonal immune complexes and microaggregated albumin as inhibitors of uptake and labeled monoclonal antibody by hepatic tissue

Conference · Wed May 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:6639500

Blend, M; Mermall, H; Pinsky, S; Frinke, J; David, G; Carlo, D

Hepatic uptake of labeled monoclonal antibody (MoAb) remains a persistent problem. Human hepatoma tumors were transplanted into adult rats and successfully imaged following the injection of In-111-antiferritin MoAb. Biodistribution studies revealed that the livers in both tumor and non-tumor bearing rats accumulate and retain labeled MoAb. Two biodegradable agents thought capable of inhibiting the uptake of MoAb by blocking the liver RES were studied. Immune complexes of human spleen ferritin and antiferritin-MoAb were prepared in vitro and tested as liver RES blocking agent to the same labeled/MoAb and labeled MoAb-ferritin complex. A preparation of microaggregated albumin with an average sphere diameter of 0.4 microns (range 0.1-5.0) was tested. These compounds were injected 30 minutes prior to the injection of labeled MoAb or MoAb:Ag complex; 2-4 rats per treatment group. All agents failed to inhibit or alter the uptake of labeled MoAb and MoAb:Ag complex at concentrations of 500 ..mu..g/rat of cold complex and 250 and 500 ..mu..g/rat of microaggregated albumin. Rats with no pretreatment showed a 38% uptake. Pretreatment with complex showed 45% uptake Microlite 43% uptake, and cold complex 46% uptake. Pretreatment with 12 mg of microaggregated albumin also failed to inhibit hepatic uptake of /sup 99m/Tc-Microlite and /sup 99m/Tc sulfur colloid. These data indicate that hepatic uptake of labeled MoAB is not the result of macrophage activity in the hepatic sinusoids.

• Research Organization: Univ. of Illinois at Chicago, IL
• OSTI ID: 6639500
• Report Number(s): CONF-850611-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 26:5
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AGGLOMERATION
ALBUMINS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIODEGRADATION
BIOLOGICAL ACCUMULATION
BODY
CATABOLISM
CHEMICAL REACTIONS
COLLOIDS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DIAGNOSIS
DIAGNOSTIC USES
DIGESTIVE SYSTEM
DISPERSIONS
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EVALUATION
EXPERIMENTAL NEOPLASMS
FERRITIN
GLANDS
HEMATOMAS
HOURS LIVING RADIOISOTOPES
IMAGES
IMMUNOASSAY
IMMUNOLOGY
INDIUM 111
INDIUM ISOTOPES
INHIBITION
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IRON COMPLEXES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIVER
MAMMALS
MAN
METABOLISM
METALLOPROTEINS
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RATS
RETENTION
RODENTS
SPLEEN
SULFUR COMPOUNDS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
TRANSPLANTS
UPTAKE
USES
VERTEBRATES
YEARS LIVING RADIOISOTOPES