Effects of chilling on protein synthesis in tomato suspension cultures
- Univ. of Guelph, Ontario (Canada)
The effect of chilling on cell growth, cell viability, protein content and protein composition in suspension cultures of L. esculentum and L. hirsutum was investigated. Cell growth for both species was arrested at 2{degrees}C but when cultures were transferred to 25{degree}C cell growth resumed. There was no difference in viability between control and chilled cultures of L. esculentum, however, L. hirsutum control cultures exhibited larger amounts of Fluorescein Diacetate induced fluorescence than chilled cultures. {sup 35}S-methionine incorporation into proteins was 2.5-2 times higher in L. hirsutum than in L. esculentum. Quantitative and qualitative differences, in {sup 35}S-methionine labelled proteins, between chilled and control cultures were observed by SDS-PAGE and fluorography. Protein content in chilled cultures decreased over time but then increased when cultures were transferred to 25{degrees}C.
- OSTI ID:
- 6639345
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
HERBS
TEMPERATURE EFFECTS
PROTEINS
BIOSYNTHESIS
CELL CULTURES
ELECTROPHORESIS
GENETIC VARIABILITY
METHIONINE
PLANT GROWTH
SULFUR 35
TOMATOES
TRACER TECHNIQUES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL VARIABILITY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FOOD
FRUITS
GROWTH
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIOISOTOPES
SULFUR ISOTOPES
SYNTHESIS
560200* - Thermal Effects
550201 - Biochemistry- Tracer Techniques