Changes in the electrical surface charge and transplantation properties of TA3 ascites tumor cells during short-term maintenance in an isotonic salt solution

Technical Report · Mon Dec 01 00:00:00 EST 1980

DOI:https://doi.org/10.2172/6637558· OSTI ID:6637558

Tenforde, T S; Richards, W R; Kelly, L S

Several experimental procedures with tumors require in vitro maintenance of the cells under conditions that represent only an approximation to the physiological milieu. Examples of such procedures include the preparation of inocula for tumor transplantation and immunological studies on tumors in a cell culture system. Another example is the in vitro - in vivo cytotoxicity test in which the proliferative capacity of tumor cells is studied in vivo following incubation with a chemotherapeutic agent in vitro. As a means of characterizing several of the degenerative processes that can occur when tumor cells are maintained in vitro under relatively nonphysiological conditions, we have examined the influence of a 0.9% NaCl suspending medium on the surface charge and transplantation properties of TA3 ascites adenocarcinoma cells.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6637558
• Report Number(s): LBL-11868
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ASCITES TUMOR CELLS
CELL PROLIFERATION
PATHOLOGICAL CHANGES
SURFACE PROPERTIES
CULTURE MEDIA
BIOLOGICAL EFFECTS
AQUEOUS SOLUTIONS
CELL CULTURES
CELL MEMBRANES
ELECTRIC CHARGES
EXPERIMENTAL DATA
IN VITRO
MICE
SALINITY
TEMPERATURE DEPENDENCE
TRANSPLANTS
ANIMAL CELLS
ANIMALS
CELL CONSTITUENTS
DATA
DISPERSIONS
INFORMATION
MAMMALS
MEMBRANES
MIXTURES
NUMERICAL DATA
RODENTS
SOLUTIONS
TUMOR CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550300 - Cytology