Characterization and localization of /sup 3/H-arginine8-vasopressin binding to rat kidney and brain tissue
Anatomic, behavioral and pharmacologic evidence suggests that arginine8-vasopressin (AVP) serves as a CNS neurotransmitter or neuromodulator. AVP binding to membrane and tissue slice preparations from brain and kidney was characterized, and the anatomical distribution of these binding sites was examined. Conditions for the binding assay were optimized using kidney medullary tissue. Binding of /sup 3/H-AVP (S.A. . 30-51 Ci/mmol, NEN) to brain and kidney membranes and tissue slices was saturable, temperature dependent, linearly related to protein concentration (or number of tissue slices), reversible, and specific since the ability of cold AVP to displace /sup 3/H-AVP from binding was greater than oxytocin and other related peptide fragments. Autoradiographic localization of /sup 3/H-AVP binding was restricted to kidney medullary tissue. In brain tissue, /sup 3/H-AVP binding was found to occur in concentrated foci. Brainstem areas such as the nucleus tractus solitarius (NTS) showed a high density of AVP binding sites. Since local injections of AVP into the NTS have been shown to influence blood pressure, the present study presents the first anatomical evidence for the presence of AVP specific binding sites which might mediate this effect.
- Research Organization:
- Veterans Administration Medical Center, Seattle, WA
- OSTI ID:
- 6630845
- Journal Information:
- Peptides (Fayetteville, N.Y.); (United States), Vol. 4:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Enhanced binding of /sup 3/H-arginine8-vasopressin in the Brattleboro rat
Electrophysiological and autoradiographical evidence of V1 vasopressin receptors in the lateral septum of the rat brain
Related Subjects
BRAIN
RECEPTORS
KIDNEYS
NEUROREGULATORS
BIOCHEMICAL REACTION KINETICS
VASOPRESSIN
ARGININE
AUTORADIOGRAPHY
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DRUGS
HORMONES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques