Continuous production of ethanol by yeast ''immobilized'' in a membrane-contained fermentor
A dialysate-feed, immobilized-cell dialysis continuous fermentation system was investigated as a method of relieving product inhibition in the conversion of glucose to ethanol by cells of Saccharomyces cerevisiae ATCC 4126. The substrate was fed into a continuous dialysate circuit and then into a batch fermentor circuit via diffusion through the microporous membranes of an intermediate dialyzer. Simultaneously, product was withdrawn from the fermentor circuit through the dialyzer membranes into the dialysate circuit and out in the effluent. Since the fermentor was operated without an effluent, the cells essentially were immobilized and converted substrate to product by maintenance metabolism. Contrary to prior results with this novel system for the continuous fermentation of lactose to lactate by lactobacillus cells, a steady state of yeast cells in the fermentor did not occur initially but was obtained by the depletion of medium nitrogen and the prevention of cell breakage, although the substrate and product concentrations then became unsteady. The inherent advantages of the system was offset in the ethanol fermentation by relatively low productivity, which appeared to be limited by membrane permeability. (Refs. 15).
- Research Organization:
- Dept of Microbiology and Public Health, Michigan State Univ, East Lansing, MI 48824
- OSTI ID:
- 6630588
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 26:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ETHANOL
PRODUCTION
FERMENTATION
ENZYME INHIBITORS
CONTINUOUS CULTURE
DIALYSIS
IMMOBILIZED CELLS
MEMBRANES
PERMEABILITY
SACCHAROMYCES CEREVISIAE
ALCOHOLS
BIOCONVERSION
FUNGI
HYDROXY COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
SACCHAROMYCES
SEPARATION PROCESSES
YEASTS
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