Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Blood coagulation factor XIa binds specifically to a site on activated human platelets distinct from that for factor XI

Journal Article · · J. Clin. Invest.; (United States)
DOI:https://doi.org/10.1172/JCI111361· OSTI ID:6626770
; ; ; ;
Binding of /sup 125/I-Factor XIa to platelets required the presence of high molecular weight kininogen, was enhanced when platelets were stimulated with thrombin, and reached a plateau after 4-6 min of incubation at 37 degrees C. Factor XIa binding was specific: 50- to 100-fold molar excesses of unlabeled Factor XIa prevented binding, whereas Factor XI, prekallikrein, Factor XIIa, and prothrombin did not. When washed erythrocytes, added at concentrations calculated to provide an equivalent surface area to platelets, were incubated with Factor XIa, only a low level of nonspecific, nonsaturable binding was detected. Factor XIa binding to platelets was partially reversible and was saturable at concentrations of added Factor XIa of 0.2-0.4 microgram/ml (1.25-2.5 microM). The number of Factor XIa binding sites on activated platelets was estimated to be 225 per platelet (range, 110-450). We conclude that specific, high affinity, saturable binding sites for Factor XIa are present on activated platelets, are distinct from those previously demonstrated for Factor XI, and require the presence of high molecular weight kininogen.
Research Organization:
Department of Medicine, Temple University School of Medicine, Philadelphia, Pennsylvania
OSTI ID:
6626770
Journal Information:
J. Clin. Invest.; (United States), Journal Name: J. Clin. Invest.; (United States) Vol. 73:6; ISSN JCINA
Country of Publication:
United States
Language:
English

Similar Records

Binding of coagulation factor XI to washed human platelets
Journal Article · Tue Jul 01 00:00:00 EDT 1986 · Biochemistry; (United States) · OSTI ID:5003156
Identification of binding sites for factors IX and IXa on human platelets
Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6024768
Kinetics of the Factor XIa catalyzed activation of human blood coagulation Factor IX
Journal Article · Tue May 01 00:00:00 EDT 1984 · J. Clin. Invest.; (United States) · OSTI ID:6749869

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CHEMICAL BONDS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HEMATOLOGIC AGENTS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RECEPTORS
TRACER TECHNIQUES