Partial proteolytic digestion of the mammary prolactin receptor: Identification of smaller prolactin binding fragments

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Journal of Receptor Research; (USA)

OSTI ID:6623254

Dusanter-Fourt, I; Kelly, P A; Djiane, J ^[1]

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1. Institut National de la Recherche Agronomique, Jouy-en-Josas (France)

Partial proteolytic digestion of the mammary prolactin (PRL) receptor was used to generate receptor fragments and analyze their immunoreactivity and PRL binding properties. Tryptic digestion of the PRL receptor produced two immunoreactive fragments (Mr approximately 30,000 and approximately 15,000) that reacted with a monoclonal anti-PRL receptor antibody and still specifically bound PRL, while the complete immunoreactive PRL binding unit (Mr approximately 42,000) disappeared. Neither chymotrypsin nor V8 protease were able to generate any immunoreactive receptor fragments. These receptor fragments may represent smaller PRL binding receptor form(s) of biological significance.

• OSTI ID: 6623254
• Journal Information: Journal of Receptor Research; (USA), Journal Name: Journal of Receptor Research; (USA) Vol. 9:6; ISSN 0197-5110; ISSN JRERD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
ENZYMES
GONADOTROPINS
HORMONES
HYDROLASES
IMMUNE REACTIONS
ISOTOPE APPLICATIONS
KINETICS
LTH
MAMMALS
MEMBRANE PROTEINS
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PITUITARY HORMONES
PROTEINS
PROTEOLYSIS
RABBITS
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES