Internal dynamics of DNA - a solid state deuterium NMR study

Miscellaneous · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6622891

Huang, Wen-Chang

In this dissertation, solid state {sup 2}H NMR spectroscopy has been used to investigate the dynamics of the sodium salt oligonucleotide, (d(CGCGAATTCGCG)){sub 2}, which contains the Eco R1 binding site. Deuterium quadrupole echo line shape and spin-lattice relaxation times were obtained as a function of hydration on three different deuterated samples. In the first sample, (d{sub 12}-(d(CG*CG*A*A*TTCG*CG*)){sub 2}), the C8 proton of all purine in the self-complementary dodecamer were exchanged for deuterons. Specifically labeled thymidine (C6 deuterated) was also synthetically incorporated at the seventh position (counting 5{prime} to 3{prime}) in the sequence (d{sub 2}-(d(CGCGAAT*TCGCG)){sub 2}). In the third sample the C2{double prime} position of the furanose ring of adenosine at the fifth and sixth positions in the same sequence (d{sub 4}-(d(CGCGA*A*TTCGCG)){sub 2}) was deuterium labeled. The static quadrupole coupling constant (e{sup 2}qQ/h) and asymmetry parameter ({eta}) were obtained through the analysis of appropriative motional models from the corresponding monomers studies.

• Research Organization: Washington Univ., Seattle, WA (USA)
• OSTI ID: 6622891
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
MOLECULAR STRUCTURE
OLIGONUCLEOTIDES
NMR SPECTRA
DEUTERIUM
NUCLEAR MAGNETIC RESONANCE
THYMIDINE
TRACER TECHNIQUES
AZINES
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RESONANCE
RIBOSIDES
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques