Localization of serotoni (5-hydroxytryptamine, 5-HT) with partial purification and characterization of a serotonin binding protein in the intestinal tissue of the nematode Ascaris suum
An intracellular 5-HT binding protein (SBP) from intestinal tissue was partially purified and characterized. Binding of ({sup 3}H) 5-HT to the protein appeared to be Fe{sup +2}-sensitive and maximal (20.8pmol/mg protein) at 5 {times} 10{sup {minus}4}M Fe{sup +2} and 10{sup {minus}7}M ({sup 3}H) 5-HT. There were two 5-HT binding sites present at optimum Fe{sup +2} concentrations. The Bmax values of these sites were more sensitive to Fe{sup +2} than Kd values. Sulfhydryl reducing agents, cation chelators, Fe{sup +3}, Ca{sup +2} and antagonists of 5-HT uptake and storage inhibited binding of 5-HT to SBP. Gel exclusion chromatography indicated the presence of a 45Kda SBP that in 5 {times} 10{sup {minus}5}M Fe{sup +2} may form aggregates ranging in size from approximately 80 to >1000Kda. The data indicate these in vitro aggregates may correspond to the electron-opaque patches observed in situ. Ascaris suum may provide a model system to further elucidate the physiological role of analogous serotonin binding proteins that have been identified in mammalian systems.
- Research Organization:
- North Texas State Univ., Denton, TX (USA)
- OSTI ID:
- 6622764
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
CHEMICAL COMPOSITION
SEROTONIN
BIOCHEMICAL REACTION KINETICS
CHELATING AGENTS
CHROMATOGRAPHY
FRACTIONATION
INTESTINES
IRON COMPOUNDS
NEMATODES
REAGENTS
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AROMATICS
ASCHELMINTHES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BODY
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
HELMINTHS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
KINETICS
MEMBRANE PROTEINS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
SEPARATION PROCESSES
SYMPATHOMIMETICS
TRANSITION ELEMENT COMPOUNDS
TRYPTAMINES
550201* - Biochemistry- Tracer Techniques