Phytochrome in photosynthetically competent plants characterization by monoclonal antibodies: Progress report

Technical Report · Sun Mar 01 04:00:00 EST 1987

OSTI ID:6618888

Pratt, L H

It is evident that phytochrome in green oats differs more markedly from that in etiolated oats than could have been anticipated. More effort has gone into characterization of green-oat phytochrome, including elimination of likely artifacts that might have been responsible for the observed differences with etiolated-oat phytochrome, and into the generation of new probes for green-oat phytochrome. The liability of green-oat phytochrome has increased the difficulty of this work. Immunochemical differences between etiolated- and green-oat phytochrome have been elucidated. A domain on green-oat phytochrome that undergoes a change upon phototransformation of at least etiolated-oat phytochrome has been discovered. A lambda gt11 cDNA library has been prepared from size-enriched poly(A)/sup +/-mRNA isolated from green oat shoots and has been screened immunochemically. An amplified ELISA protocol suitable for screening hybridoma supernatants against crude green-oat phytochrome preparations has been developed. Monoclonal antibodies directed to green-oat phytochrome have been identified. 5 refs.

• Research Organization: Georgia Univ., Athens (USA). Dept. of Botany
• DOE Contract Number: AS09-81ER10925
• OSTI ID: 6618888
• Report Number(s): DOE/ER/10925-5; ON: DE87009342
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
CEREALS
CHROMATOGRAPHY
ENZYME IMMUNOASSAY
FRACTIONATION
GRASS
IMMUNOASSAY
LIQUID COLUMN CHROMATOGRAPHY
MONOCLONAL ANTIBODIES
OATS
ORGANIC COMPOUNDS
PHYTOCHROMES
PIGMENTS
PLANTS
PROTEINS
SEPARATION PROCESSES