Sources of propionate for the biogenesis of ethyl-braced insect juvenile hormones: role of isoleucine and valine

Journal Article · Sun Nov 01 04:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.22.7906· OSTI ID:6618810

Brindle, P A; Baker, F C; Tsai, L W; Reuter, C C

Corpora allata from adult female Manduca sexta biosynthesis the sesquiterpenoid juvenile hormone (JH) III and the unusual ethyl-branched homologue JH II in vitro. The authors maintained corpora allata in medium 199 using (methyl-/sup 3/H)methionine as the source of the JH methyl ester moiety and as a mass marker. This allowed measurement of the relative contributions of /sup 14/C-labeled precursors to the biogenesis of JH II and III carbon skeletons. They showed efficient incorporation of a propionate equivalent, from isoleucine or valine catabolism, into the ethyl-branched portion of JH II, using double-label liquid scintillation counting of isolated JHs and gas chromatography/mass spectrometry with selected ion monitoring of JH deuteromethoxyhydrin derivatives. Methionine was a poor source of propionate for JH II biosynthesis, while glucose, succinate, threonine, and ..beta..-alanine did not contribute propionate at all. Leucine, isoleucine, and glucose incorporated into JH III and the acetate-derivative portion of JH II.

• Research Organization: Zoecon Research Institute, Palo Alto, CA (USA)
• OSTI ID: 6618810
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:22; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALANINES
ALDEHYDES
AMINO ACIDS
ANIMALS
ARTHROPODS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DRUGS
GAS CHROMATOGRAPHY
GLUCOSE
HEXOSES
HORMONES
HYDROXY ACIDS
INSECTS
INVERTEBRATES
JUVENILES
LABELLED COMPOUNDS
LEUCINE
LIPOTROPIC FACTORS
MASS SPECTROSCOPY
METHIONINE
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROPIONIC ACID
SACCHARIDES
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
THREONINE
TRITIUM COMPOUNDS
VALINE