Characterization of the apolipoprotein AI and CIII genes in the domestic pig

Journal Article · Mon Mar 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1119· OSTI ID:6616369

Birchbauer, A; Knipping, G; Juritsch, B; Zechner, R ^[1]; Aschauer, H ^[2]

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1. Univ. of Graz (Austria)
2. Sandoz-Forschungs Institut Ges.m.b.H., Vienna (Austria)

The apolipoproteins (apo) AI and CIII are important constituents of triglyceride-rich lipoproteins and high-density lipoproteins. In humans, apo AI is believed to play an important protective role in the pathogenesis of arteriosclerosis, whereas apo CIII might be involved in the development of hypertriglyceridemia. Both human genes are located within a gene cluster on chromosome 11. Although the domestic pig has been widely used as an animal model in arteriosclerosis and lipid research, the porcine apolipoproteins genes are poorly characterized. In this report, the complete nucleotide sequences of the porcine apo AI and CIII genes are presented and the authors demonstrate, for the first time, apo CIII expression in the pig. Both genes are composed of four exons and three introns and resemble closely their human counterparts with regard to the transcriptional start sites, exon sizes, intron sizes, exon-intron borders, and the size of the intergenic region. The predicted pig apo AI is a protein of 241 amino acids, which is 2 amino acids shorter than human apo AI. The protein sequence was found to be very homologous to apo AI sequences in other mammalian species. Apo AI expression was detected on the mRNA level in porcine liver and intestine. The apo CIII gene encodes a protein with 73 amino acids, which is 6 amino acids shorter than human apo CIII. In contrast to the three isoforms of apo CIII found in humans, only one major isoform was detected in the pig. Presumably this isoform is unglycosylated. In addition to apo CIII expression in the liver and the intestine, a truncated form of apo CIII mRNA was also found in porcine kidney. The studies demonstrate the presence of an apo CIII gene, an apo CIII mRNA, and an apo CIII protein in the pig and, therefore, exclude a hypothesized apo CIII deficiency in these animals. 53 refs., 5 figs.

• OSTI ID: 6616369
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:3; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
APOLIPOPROTEINS
ARTERIOSCLEROSIS
BIOLOGICAL MODELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CHROMOSOMES
DIGESTIVE SYSTEM
DISEASES
DOMESTIC ANIMALS
GENES
GLANDS
HUMAN CHROMOSOMES
LIPIDS
LIPOPROTEINS
LIVER
MAMMALS
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PROTEINS
SIZE
SWINE
VASCULAR DISEASES
VERTEBRATES