Survival and antigenic profile of irradiated malarial sporozoites in infected liver cells

Journal Article · Sat Sep 01 04:00:00 EDT 1990 · Infection and Immunity; (USA)

OSTI ID:6616191

Suhrbier, A; Winger, L A; Castellano, E; Sinden, R E ^[1]

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1. Imperial College, London (England)

Exoerythrocytic (EE) stages of Plasmodium berghei derived from irradiated sporozoites were cultured in vitro in HepG2 cells. They synthesized several antigens, predominantly but not exclusively those expressed by normal early erythrocytic schizonts. After invasion, over half the intracellular sporozoites, both normal and irradiated, appeared to die. After 24 h, in marked contrast to the normal parasites, EE parasites derived from irradiated sporozoites continued to break open, shedding their antigens into the cytoplasm of the infected host cells. Increasing radiation dosage, which has previously been shown to reduce the ability of irradiated sporozoites to protect animals, correlated with reduced de novo antigen synthesis by EE parasites derived from irradiated sporozoites.

• OSTI ID: 6616191
• Journal Information: Infection and Immunity; (USA), Journal Name: Infection and Immunity; (USA) Vol. 58:9; ISSN 0019-9567; ISSN INFIB
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CELL CONSTITUENTS
CYTOPLASM
DIGESTIVE SYSTEM
DOSES
GLANDS
INVERTEBRATES
LIVER
MICROORGANISMS
ORGANS
PARASITES
PLASMODIUM
PROTOZOA
RADIATION DOSES
RADIATION EFFECTS
SPOROZOA
SYNTHESIS
TUMOR CELLS