Effect of DNA sequence, ionic strength, and cationic DNA affinity binders on the methylation of DNA by N-methyl-N-nitrosourea

Journal Article · · Chem. Res. Toxicol.; (United States)

DOI:https://doi.org/10.1021/tx00003a003· OSTI ID:6615375

Wurdeman, R L; Gold, B

DNA alkylation by N-alkyl-N-nitrosoureas is generally accepted to be responsible for their mutagenic, carcinogenic, and antineoplastic activities. The exact nature of the ultimate alkylating intermediate is still controversial, with a variety of species having been nominated. The sequence specificity for DNA alkylation by simple N-alkyl-N-nitrosoureas has not been reported, although such information is basic in understanding the specific point mutations induced by these compounds in oncogene targets. These two points are addressed by using N-methyl-N-nitrosourea methylation of a 576 base-pair /sup 32/P-end-labeled DNA restriction fragment and high-resolution polyacrylamide sequencing gels. This method provides information on the formation of N/sup 7/-methylguanine, by the generation of single-strand breaks upon exposure to piperidine.

• Research Organization: Univ. of Nebraska Medical Center, Omaha (USA)
• OSTI ID: 6615375
• Journal Information: Chem. Res. Toxicol.; (United States), Vol. 1:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
DNA
METHYLATION
NITROSOUREAS
GENETIC EFFECTS
DNA SEQUENCING
ELECTROPHORESIS
MUTATIONS
PHOSPHORUS 32
SPECIFICITY
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NITROSO COMPOUNDS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques