SR-2508 plus buthionine sulfoximine or SR-2508 alone: effects on the radiation response and the glutathione content of a human tumor xenograft

Journal Article · Wed Apr 01 00:00:00 EST 1987 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576892· OSTI ID:6613517

Lespinasse, F; Biscay, P; Malaise, E P; Guichard, M

This study determined the radiosensitivity of the human tumor xenograft HT29 and its glutathione (GSH) and cysteine (CYS) contents after treatment with both buthionine sulfoximine (BSO) and SR-2508 or SR-2508 alone. Tumor radiosensitivity was assessed by the in vitro colony assay and thiol content was measured by high-performance liquid chromatography. The radiosensitizing effect of SR-2508 is dose dependent and increases when higher doses of radiation are given. SR-2508 given alone does not modify GSH and CYS content; however, when given with BSO, the GSH level is significantly reduced, yet radiosensitivity of the HT29 tumor is only slightly increased. These results have been compared to our previously observed results of HT29 treatment with misonidazole (MISO), BSO, or MISO + BSO.

• Research Organization: Institut Gustave-Roussy, Villejuif, France
• OSTI ID: 6613517
• Journal Information: Radiat. Res.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
NEOPLASMS
RADIOSENSITIVITY
TRANSPLANTS
RADIOSENSITIZERS
RADIOSENSITIVITY EFFECTS
CYSTEINE
DOSE-RESPONSE RELATIONSHIPS
GLUTATHIONE
LIQUID COLUMN CHROMATOGRAPHY
MAN
AMINO ACIDS
ANIMALS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DISEASES
DRUGS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
SEPARATION PROCESSES
THIOLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture