Quantitative X-ray microanalysis of biological specimens

Journal Article · Sun May 01 04:00:00 EDT 1988 · J. Electron Micros. Tech.; (United States)

DOI:https://doi.org/10.1002/jemt.1060090104· OSTI ID:6611482

Roomans, G M

Qualitative X-ray microanalysis of biological specimens requires an approach that is somewhat different from that used in the materials sciences. The first step is deconvolution and background subtraction on the obtained spectrum. The further treatment depends on the type of specimen: thin, thick, or semithick. For thin sections, the continuum method of quantitation is most often used, but it should be combined with an accurate correction for extraneous background. However, alternative methods to determine local mass should also be considered. In the analysis of biological bulk specimens, the ZAF-correction method appears to be less useful, primarily because of the uneven surface of biological specimens. The peak-to-local background model may be a more adequate method for thick specimens that are not mounted on a thick substrate. Quantitative X-ray microanalysis of biological specimens generally requires the use of standards that preferably should resemble the specimen in chemical and physical properties. Special problems in biological microanalysis include low count rates, specimen instability and mass loss, extraneous contributions to the spectrum, and preparative artifacts affecting quantitation. A relatively recent development in X-ray microanalysis of biological specimens is the quantitative determination of local water content.

• Research Organization: Univ. of Stockholm (Sweden)
• OSTI ID: 6611482
• Journal Information: J. Electron Micros. Tech.; (United States), Journal Name: J. Electron Micros. Tech.; (United States) Vol. 9:1; ISSN JEMTE
• Country of Publication: United States
• Language: English

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