Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Effect of isotype on the biodistribution of radioiodinated immunoglobulins

Conference · · J. Nucl. Med.; (United States)
OSTI ID:6604736
; ; ; ; ; ; ; ;
A series of monoclonal antibodies of different isotypes developed against the same arsenate hapten were labeled by the iodogen bead method and employed to define the importance of isotype on biodistribution. Studies were performed with IgG1, IgG2a, IgG2b, IgG3, and IgM (pentamer). An ELISA assay performed before and after labeling demonstrated retained antigen combining activity. Groups of 6 rats each were injected with 12-18 ..mu..g/kg antibody and killed 1.5, 24, 48, 72 and 144 hours later. The concentration of radio-iodine in blood, heart, lung, liver, spleen, kidney, stomach, GI tract, and skeletal muscle were determined and expressed as a percent injected dose/organ. The mean half-time of blood clearance was less than 24 hours for all isotypes. Retention in the blood at 24 hours averaged 28% of the injected dose/organ for the IgG but only 9.4% with IgM. No focal localization was observed in any organ. Whole body counts indicated a 2 component clearance with an initial half-time between 48 and 72 hours for IgG, while IgM had a half-time of less than 24 hours. These data suggest that IgG isotypes have similar biodistributions, while IgM has a shorter biological half-time.
Research Organization:
Massachusetts General Hospital, Boston, MA
OSTI ID:
6604736
Report Number(s):
CONF-850611-
Conference Information:
Journal Name: J. Nucl. Med.; (United States) Journal Volume: 26:5
Country of Publication:
United States
Language:
English

Similar Records

Human anti-murine immune response following administration of radiolabeled monoclonal antibodies
Conference · Wed May 01 00:00:00 EDT 1985 · J. Nucl. Med.; (United States) · OSTI ID:5874019
Biokinetics of a radioiodinated antibreast carcinoma monoclonal antibody and fragment in humans
Conference · Sat Dec 31 23:00:00 EST 1983 · J. Nucl. Med.; (United States) · OSTI ID:6593470
Biodistribution, pharmacokinetic, and imaging studies with sup 186 Re-labeled NR-LU-10 whole antibody in LS174T colonic tumor-bearing mice
Journal Article · Fri Dec 14 23:00:00 EST 1990 · Cancer Research; (USA) · OSTI ID:6070146

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
ARSENATES
ARSENIC COMPOUNDS
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CLEARANCE
COMPARATIVE EVALUATIONS
COMPUTER CODES
DIAGNOSTIC USES
DIGESTIVE SYSTEM
DISTRIBUTION
E CODES
GASTROINTESTINAL TRACT
GLANDS
GLOBULINS
HALF-LIFE
HALOGENATION
HEART
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
INJECTION
INTAKE
IODINATION
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LABELLING
LIVER
LUNGS
MAMMALS
MATERIALS
MONOCLONAL ANTIBODIES
MUSCLES
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PROTEINS
QUANTITY RATIO
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RESPIRATORY SYSTEM
RETENTION
RODENTS
SPLEEN
STOMACH
TISSUE DISTRIBUTION
TRACER TECHNIQUES
USES
VERTEBRATES