Cytochrome C oxidase activity in germinating Phaseolus vulgaris l. seeds: Effects of carbon monoxide
Journal Article
·
· Plant Physiology, Supplement; (USA)
OSTI ID:6604197
- Colorado State Univ., Fort Collins (USA)
Cytochrome c oxidase is a key bioenergetic enzyme required for seed germination. The enzyme was isolated from 2-day germinating beans and biochemically compared to its bovine heart counterpart. Carbon monoxide, which binds to the heme a{sub 3} site of cytochrome c oxidase, we used to probe O{sub 2} utilization activity in isolated enzyme, mitochondrial particles, and whole seeds. Bean seeds under 80% CO/20% O{sub 2} exhibited 46% growth inhibition as determined by root length. Reversible, dose-dependent partial inhibition of bean seed mitochondrial respiration was observed in the presence of CO; heart mitochondria had a more sensitive, less reversible response. Effects of CO on bean and bovine heart enzyme were similar. The close correlation of CO effects observed on seedling growth, mitochondrial respiration and cytochrome oxidase activity indicate an important role for this enzyme during the early stages of seed germination.
- OSTI ID:
- 6604197
- Journal Information:
- Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CARDIOVASCULAR SYSTEM
CATTLE
CELL CONSTITUENTS
CHALCOGENIDES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
GERMINATION
HEART
INHIBITION
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MAMMALS
MITOCHONDRIA
MIXED-FUNCTION OXIDASES
ORGANOIDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
PHASEOLUS
PLANTS
RUMINANTS
SEEDS
SPROUT INHIBITION
TOXICITY
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CARDIOVASCULAR SYSTEM
CATTLE
CELL CONSTITUENTS
CHALCOGENIDES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
GERMINATION
HEART
INHIBITION
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MAMMALS
MITOCHONDRIA
MIXED-FUNCTION OXIDASES
ORGANOIDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
PHASEOLUS
PLANTS
RUMINANTS
SEEDS
SPROUT INHIBITION
TOXICITY
VERTEBRATES