Regulatory mechanisms in Thielavia terrestris. Final report

Technical Report · Mon Mar 15 04:00:00 EST 1982

OSTI ID:6603951

Tuse, D; Hokama, L

Thielavia terrestris is a filamentous ascomycete originally discovered and identified as Allescheria terrestris by Apinis (1963). Strain S-16 was isolated from a soil sample in California and found to grow at temperatures as high as 50/sup 0/C. The fungus produces a complete cellulase system that enables it to degrade native, crystalline cellulose to glucose. The enzymes display high heat and pH stability and an optimum temperature for hydrolysis of 65/sup 0/C, making it attractive for the eventual commercial conversion of cellulose into fuel and chemicals. We report on the enzyme activity of Thielavia terrestris S-16, the effects of medium composition and carbon source on enzyme production, the growth of the organism at different temperatures, the extracellular aryl-..beta..-glucosidase activitiy as a function of incubation temperature, the effects of small-molecular-weight metabolic regulators and membrane-active antibiotics on the cell-associated and extracellular enzyme levels, and the methods used in an attempt to find plasmids.

• Research Organization: SRI International, Menlo Park, CA (USA). Biotechnology Research Dept.
• DOE Contract Number: AC03-80ER10697
• OSTI ID: 6603951
• Report Number(s): DOE/ER/10697-T1; ON: DE83002858
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBOHYDRATES
CELLULOLYTIC ACTIVITY
CELLULOSE
CULTURE MEDIA
ENZYME ACTIVITY
FUNGI
METABOLISM
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
RESPONSE MODIFYING FACTORS
SACCHARIDES
SYNTHESIS
TEMPERATURE DEPENDENCE
TEMPERATURE EFFECTS