Amino acid metabolism of Lemna minor L

Journal Article · Fri Mar 31 23:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.89.4.1161· OSTI ID:6602266

Rhodes, D; Rich, P J; Brunk, D G ^[1]

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1. Purdue Univ., West Lafayette, IN (USA)

A serious limitation to the use of N(O,S)-heptafluorobutyryl isobutyl amino acid derivatives in the analysis of {sup 15}N-labeling kinetics of amino acids in plant tissues, is that the amides glutamine and asparagine undergo acid hydrolysis to glutamate and aspartate, respectively, during derivatization. This led us to consider an alternative procedure for derivatization of glutamine and asparagine with N-methyl-N-(tert-butyldimethylsilyl)-trifluoroacetamide in pyridine. Gas chromatography-mass spectrometry yielded fragment ions (M-57) of mass 417 and 431 for the ({sup 14}N)asparagine and ({sup 14}N)glutamine derivatives, respectively, suitable for monitoring unlabeled, single-{sup 15}N- and double-{sup 15}N-labeled amide species from the ion clusters at mass to charge ratio (m/z) 415 to 423 for asparagine, and m/z 429 to 437 for glutamine. From separate analyses of the specific isotope abundance of the amino-N groups of asparagine and glutamine as their N-heptafluorobutyryl isobutyl derivatives, the specific amide-({sup 15}N) abundance of these amino acids was determined.

• OSTI ID: 6602266
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 89:4; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
ASPARAGINE
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
GAS CHROMATOGRAPHY
GLUTAMINE
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MASS SPECTROSCOPY
METABOLISM
NITROGEN 14
NITROGEN 15
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANT TISSUES
PLANTS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
TISSUES
TRACER TECHNIQUES