Mechanism of action of the copper(I) complex of 2,9-dimethyl-1,10-phenanthroline on Mycoplasma gallisepticum
Evidence was found that the inhibitory action of Cu(DMP)/sub 2/NO/sub 3/, the copper(I) complex of 2,9-dimethyl-1,10-phenanthroline (DMP), on Mycoplasma gallisepticum is a consequence of the ultimate toxicity of copper, and not that of the ligand, DMP. From uptake studies with radiolabeled /sup 67/Cu and (/sup 14/C)DMP, we concluded that significantly more copper than DMP is bound to the mycoplasmal cell. It appeared that dissociation of Cu(DMP)2+ occurred shortly after interaction with the cell membrane. Copper was transported across the cytoplasmic membrane. A strong dependence of copper uptake on the incubation medium was observed in the absence of DMP. The main function of the ligand DMP appeared to be as a vehicle for the transport of copper from nontoxic copper-medium complexes to membrane-buried cellular ligands.
- Research Organization:
- Departments of Medicinal Chemistry, Universiteit, Amsterdam, The Netherlands
- OSTI ID:
- 6601774
- Journal Information:
- Antimicrob. Agents Chemother.; (United States), Journal Name: Antimicrob. Agents Chemother.; (United States) Vol. 21:6; ISSN AMACC
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTI-INFECTIVE AGENTS
ANTIMICROBIAL AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
COMPLEXES
COPPER 67
COPPER COMPLEXES
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGANDS
MEMBRANE TRANSPORT
MICROORGANISMS
MYCOPLASMA
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
SENSITIVITY
TOXICITY
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
UPTAKE