Effect of temperature on consecutive denitrification reactions in Brookston clay and Fox sandy loam

Journal Article · Tue May 01 00:00:00 EDT 1984 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6601492

McKenney, D J; Johnson, G P; Findlay, W I

The kinetics of several steps in the microbial denitrification process in Brookston clay and Fox sandy loam, two soils common to Southwestern Ontario, were studied in the temperature range of 5 to 25/sup 0/C. The extent of chemical denitrification was also determined in otherwise identical but sterilized soils at temperatures up to 80/sup 0/C. A gas flow system was used in which soil gases were continuously removed from anaerobic soil columns by argon carrier gas. Net steady-state rates of NO and N/sub 2/O production, rates of loss of NO/sub 3//sup -/, and production and loss of NO/sub 2//sup -/ were measured over periods of up to 5 days. Arrhenius activation energies for the zero-order process NO/sub 3//sup -/ ..-->.. NO/sub 2//sup -/ were calculated to be 50 +/- 9 KJ mol/sup -1/ for Brookston clay and 55 +/- 13 KJ mol/sup -1/ for Fox sandy loam. The overall reaction, NO/sub 2//sup -/ ..-->.. NO (chemodenitrification), in both sterile soils was accurately first order with respect to NO/sub 2//sup -/; the activation energy was 70 +/- 2.8 kJ mol/sup -1/ in Brookston clay and 79 +/- 1.2 kJ mol/sup -1/ in the sandy loam, and the preexponential factors were (2.3 +/- 1.2) x 10/sup 9/ and (5.7 +/- 1.2) x 10/sup 9/ min/sup -1/, respectively. 21 references.

• Research Organization: Univ. of Windsor, Ontario
• OSTI ID: 6601492
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 47:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
DENITRIFICATION
TEMPERATURE EFFECTS
SOILS
ACTIVATION ENERGY
ANAEROBIC CONDITIONS
BIODEGRADATION
KINETICS
MICROORGANISMS
NITRATES
NITROGEN OXIDES
SOIL CHEMISTRY
CHALCOGENIDES
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
ENERGY
NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
550700* - Microbiology
510100 - Environment
Terrestrial- Basic Studies- (-1989)