Radiosensitivity and thermosensitization of thermotolerant Chinese hamster cells and RIF-1 tumors

Journal Article · Sun Jul 01 00:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576455· OSTI ID:6600802

Hartson-Eaton, M; Malcolm, A W; Hahn, G M

CHO cells subline HA-1 were made thermotolerant by a priming heat treatment(43/sup 0/C, 30 min). Later, 4, 16, or 24 hr, they were either irradiated or heated (43/sup 0/C, 30 min) and irradiated. Thermotolerance had no effect on the radiation sensitivity of the cells as measured by the D/sub 0/ value of the clonogenic survival curve. However the N value of the curve (width of shoulder) showed a significant increase at 24 hr, indicating an increased capacity to accumulate sublethal damage. The same priming treatment was given to RIF-1 tumors growing in C3H mice. Later, 24 hr, when the tumors were either irradiated or heated (43/sup 0/C, 30 min) and irradiated, it was found that thermotolerance had no effect on the radiosensitivity of the cells as measured by in vitro assay. However, thermal radiosensitization was not apparent 24 hr after the priming treatment.

• Research Organization: Vanderbilt Univ. Hospital, Nashville, TN
• OSTI ID: 6600802
• Journal Information: Radiat. Res.; (United States), Vol. 99:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL KILLING
RADIOINDUCTION
CHO CELLS
BIOLOGICAL RADIATION EFFECTS
TEMPERATURE EFFECTS
EXPERIMENTAL NEOPLASMS
HYPERTHERMIA
RADIOSENSITIVITY EFFECTS
MICE
STATISTICS
SURVIVAL CURVES
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY TEMPERATURE
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
MATHEMATICS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
560201 - Thermal Effects- Cells- (-1987)