Expression of Escherichia coli uvr genes in mammalian cells. Progress report
Technical Report
·
OSTI ID:6598661
The goal was to determine if Escherichia coli uvr genes could be expressed in repair deficient mammalian cells (Xeroderma pigmentosum). Progress is reported in the following areas: (1) ATPase activity was assessed in which the release of labelled orthophosphate from ..gamma..-(/sup 32/P)-ATP is effected by damaged DNA and the uvrB protein; (2) the binding of the uvrA protein to damaged DNA was tested; and (3) a double uvrA and uvrC vector was engineered with gpt and the gpt/sup +/ clones isolated. 2 references, 2 figures. (ACR)
- Research Organization:
- Johns Hopkins Univ., Baltimore, MD (USA). School of Hygiene and Public Health
- DOE Contract Number:
- AC02-76EV02814
- OSTI ID:
- 6598661
- Report Number(s):
- DOE/EV/02814-6; ON: DE84016154
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550700* -- Microbiology
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ATP
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ESCHERICHIA COLI
GENES
GENETIC ENGINEERING
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PROTEINS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
RECOVERY
REPAIR
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
XP CELLS
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ATP
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ESCHERICHIA COLI
GENES
GENETIC ENGINEERING
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PROTEINS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
RECOVERY
REPAIR
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
XP CELLS