Improved double-vial radiorespirometric technique for mineralization of /sup 14/C-labeled substrates

Journal Article · Tue May 01 04:00:00 EDT 1984 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6598532

Coveney, M F; Wetzel, R G

New procedures which simplify sample preparation and improve counting efficiency were developed for double-vial radiorespirometry. Under certain conditions, efficiency of fluor-NaOH-impregnated wicks was not stable, but was adversely affected by water vapor, NaOH concentration, and CO/sub 2/ loading. Glass fiber wick material treated with a methanolic fluor-NaOH solution showed improved /sup 14/C counting efficiency (58%) compared with previous methods.

• Research Organization: Michigan State Univ., Hickory Corners
• OSTI ID: 6598532
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 47:5; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CLEARANCE
COUNTING TECHNIQUES
EXCRETION
EXHALATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID SCINTILLATION DETECTORS
MEASURING INSTRUMENTS
MEASURING METHODS
MICROORGANISMS
OXIDES
OXYGEN COMPOUNDS
RADIATION DETECTORS
RESPIRATION
SAMPLE PREPARATION
SCINTILLATION COUNTERS
SCINTILLATION COUNTING
TRACER TECHNIQUES