Isolation and characterization of a human pseudogene for the regulatory subunit RI[alpha] of cAMP-dependent protein kinases and its sublocalization on chromosome 1
- Univ. of Oslo (Norway) Rikshospitalet, Oslo (Norway)
- Rikshospitalet, Oslo (Norway)
Using a human cDNA (complementary DNA) encoding the regulatory subunit RI[alpha] of cAMP-dependent protein kinases (PKA) as a probe, a pseudogene for this PKA isoform was isolated from a human genomic library. The human RI[alpha] pseudogene was 89% similar to the open reading frame of the expressed human RI[alpha] at the nucleotide level. Several stop codons were found, indicating that the pseudogene does not encode a functional protein. The pseudogene also contained several frameshift mutations, small deletions, and insertions. No introns were found in the region corresponding to the open reading frame of the expressed RI[alpha] cDNA. Specific oligonucleotides for the RI[alpha] gene and pseudogene were constructed and used as primers in polymerase chain reaction (PCR) to amplify human DNA from healthy blood donors. Probing of the PCR products using oligonucleotides specific for the RI[alpha] gene and RI[alpha] pseudogene, respectively, showed the presence of both genes in the human genome. When DNA extracted from various somatic cell hybrids was amplified, it was shown that the RI[alpha] gene was located on human chromosome 17, whereas the RI[alpha] pseudogene was located in the P21-p31 region on chromosome 1. This is the first report describing a pseudogene for a subunit of cAMP-dependent protein kinases from any species. 30 refs., 5 figs., 1 tab.
- OSTI ID:
- 6597064
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:3; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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