Immobilization of enzyme into poly(vinyl alcohol) membrane
Journal Article
·
· Biotechnol. Bioeng.; (United States)
Glucoamylase, invertase, and cellulase were entrapped within poly(vinyl alcohol) (PVA) membrane cross-linked by means of irradiation of ultraviolet light. The conditions for immobilization of glucoamylase were examined with respect to enzyme concentration in PVA, sensitizer (sodium benzoate) concentration in PVA, irradiation time, and membrane thickness. Various characteristics of immobilized glucoamylase were evaluated. Among them, the pH activity curve for the immobilized enzyme was superior to that for the native one, and thermal stability was improved by immobilization with bovine albumin. The apparent Km was larger for immobilized glucoamylase than for the native one, while Vmax was smaller for the immobilized enzyme. Also, the apparent Km appeared to be affected by the molecular size of the substrate. Further, immobilized invertase and cellulase showed good stabilities in repeating usage. 9 references.
- Research Organization:
- Technological Univ. of Nagaoka, Niigata, Japan
- OSTI ID:
- 6593822
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 28:11; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ALKALI METAL COMPOUNDS
ANIMALS
CATTLE
CELLULASE
CHEMICAL PREPARATION
DIAGRAMS
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ENZYMES
EVALUATION
GLYCOSYL HYDROLASES
HYDROLASES
IMMOBILIZED ENZYMES
MAMMALS
MEMBRANES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PH VALUE
PHYSICAL PROPERTIES
POLYMERS
POLYVINYLS
PROTEINS
QUANTITY RATIO
RADIATIONS
RUMINANTS
SIZE
SODIUM COMPOUNDS
STABILITY
SUBSTRATES
SYNTHESIS
THERMODYNAMIC PROPERTIES
ULTRAVIOLET RADIATION
VERTEBRATES
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ALKALI METAL COMPOUNDS
ANIMALS
CATTLE
CELLULASE
CHEMICAL PREPARATION
DIAGRAMS
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ENZYMES
EVALUATION
GLYCOSYL HYDROLASES
HYDROLASES
IMMOBILIZED ENZYMES
MAMMALS
MEMBRANES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PH VALUE
PHYSICAL PROPERTIES
POLYMERS
POLYVINYLS
PROTEINS
QUANTITY RATIO
RADIATIONS
RUMINANTS
SIZE
SODIUM COMPOUNDS
STABILITY
SUBSTRATES
SYNTHESIS
THERMODYNAMIC PROPERTIES
ULTRAVIOLET RADIATION
VERTEBRATES