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Limited and selective transfer of plasma membrane glycoproteins to membrane of secondary lysosomes

Journal Article · · J. Cell Biol.; (United States)
;
Radioactive galactose, covalently bound to cell surface glycoconjugates on mouse macrophage cells, P388D/sub 1/, was used as a membrane marker to study the composition, and the kinetics of exchange, of plasma membrane-derived constituents in the membrane of secondary lysosomes. Secondary lysosomes were separated from endosomes and plasma membrane by self-forming Percoll density gradients. Horseradish peroxidase, taken up by fluid-phase pinocytosis, served as a vesicle contents marker to monitor transfer of endosomal contents into secondary lysosomes. Concurrently, the fraction of plasma membrane-derived label of secondary lysosomes increased by first order kinetics from <0.1% to a steady-state level of approx.2.5% of the total label. As analyzed by NaDodSO/sub 4/ PAGE, labeled molecules of M/sub r/ 160-190 kD were depleted and of the M/sub r/ 100-120 kD were enriched in lysosome membrane compared with the relative composition of label on the cell surface. No corresponding selectivity was observed for the degradation of label, with all M/sub r/ classes being affected to the same relative extent. The results indicate that endocytosis-derived transfer of plasma membrane constitutents to secondary lysosomes is a limited and selective process, and that only approx.1% of internalized membrane is recycled via a membrane pool of secondary lysosomes.
Research Organization:
Council for Scientific and Industrial Research, Pretoria, South Africa
OSTI ID:
6590397
Journal Information:
J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 103:4; ISSN JCLBA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CELL CONSTITUENTS
CELL MEMBRANES
CHALCOGENIDES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DECOMPOSITION
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
GALACTOSE
GLYCOLIPIDS
HEXOSES
HYDROLYSIS
LABELLED COMPOUNDS
LIPIDS
LYSIS
LYSOSOMES
MACROPHAGES
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
MICE
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANOIDS
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
PHAGOCYTOSIS
RODENTS
SACCHARIDES
SOLVOLYSIS
SOMATIC CELLS
TRITIUM COMPOUNDS
TRITIUM OXIDES
VERTEBRATES