Radiation dosimetry in experimental animals exposed to tritiated water under different conditions. [Comparison between single injection and protracted low-level exposures in mice]

Conference · Sun Jan 01 04:00:00 EST 1978

OSTI ID:6589467

Cumming, R B; Sega, G A; Walton, M F

The radiation dose to the germ cells of male mice, which sired the offspring scored in a specific-locus mutation test of injected tritiated water, was calculated. The weighted mean dose for germ cells which received all of the radiation at postspermatogonial stages was 430 rads, while that for germ cells irradiated almost entirely as spermatogonia was 615 rads. Most of the animals received a single intraperitoneal injection of HTO of 0.50 mCi/g body weight, but a few of them received 00.75 mCi/g. These weighted mean doses reflect the contribution of both groups. The percentage of the total dose delivered from /sup 3/H incorporated into macromolecules is small - less than 0.5%. The percentage of the total radioactivity in dry material from the testis on day 1 postinjection is 0.6%, on day 7 it is 3.2%, and on day 605 it is 95%. Tritium is incorporated into testicular DNA from tritiated water, and peak levels of radioactivity in this macromolecule are reached from 3 to 9 days following injection. The stable incorporation of tritium into trichloracetic acid insoluble materials is about 75% in protein and 25% in nucleic acids at all time periods following injection. Doses from single injections of tritiated water are inherently more variable than for protracted low-level exposures. This is because small differences in water balance near the time of injection can make a very large difference in total radiation dose.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6589467
• Report Number(s): CONF-781029-7
• Country of Publication: United States
• Language: English

Related Subjects

560162* -- Radionuclide Effects
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& Toxicology-- Animals
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Microorganisms
& Cells
560171 -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
COMPARATIVE EVALUATIONS
DNA
DOSES
DOSIMETRY
GERM CELLS
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
IRRADIATION PROCEDURES
LABELLED COMPOUNDS
MALES
MAMMALS
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RADIATION DOSES
RODENTS
SINGLE INTAKE
TRITIUM COMPOUNDS
TRITIUM OXIDES
UPTAKE
VERTEBRATES