Neoplastic transformation of rat mammary cells exposed to 7,12-dimethylbenz(a)anthracene or N-nitrosomethylurea in cell culture

Journal Article · Tue Aug 01 04:00:00 EDT 1978 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.75.8.3836· OSTI ID:6589176

Richards, J; Nandi, S

Primary cultures of mammary cells from virgin Lewis rats were seeded at 5 x 10/sup 5/ cells per cm/sup 2/ in medium 199 supplemented with 10% fetal calf serum, insulin (5 ..mu..g/ml), prolactin (5 ..mu..g/ml), estradiol (5 ng/ml), progesterone (0.5 ..mu..g/ml), and hydrocortisone (0.5 ..mu..g/ml). On the second or third day of culture, cells were exposed to either 7,12-dimethylbenz(a)anthracene (0.1 ..mu..g/ml for 24 hr) or N-nitrosomethylurea (80 ..mu..g/ml for 2 hr). The cells were later assayed for transformation by transplanting 10/sup 6/ or 10/sup 5/ cells into gland-free mammary fat pads of 3-week-old female hosts. Untreated cells produced only normal mammary outgrowths when transplanted. Cells treated with 7,12-dimethylbenz(a)anthracene or N-nitrosomethylurea produced abnormal outgrowths in 11% of the transplants. These abnormal outgrowths ranged from rapidly growing adenocarcinoma to alveolar and ductal hyperplastic lesions. The results indicate that rat mammary epithelial cells can be transformed by exposure to chemical carcinogens in culture and thus represent a potenial in vitro model for epithelial cell transformation.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 6589176
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 75:8; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BODY
CELL CULTURES
DISEASES
GLANDS
MAMMALS
MAMMARY GLANDS
MORPHOLOGICAL CHANGES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RATS
RODENTS
TRANSPLANTS
VERTEBRATES