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Influence of Rhodococcus equi on the respiratory burst of resident alveolar macrophages from horses

Thesis/Dissertation ·
OSTI ID:6586467

Rhodococcus equi is the etiologic agent of a devastating pneumonia of sporadic incidence in foals. The purpose of this study was to evaluate the influence of R. equi on the superoxide anion production, measured spectrophotometrically as the reduction of cytochrome C, and hexose monophosphate shunt activity, measured by /sup 14/CO/sub 2/ liberation from /sup 14/C-1-D-glucose, of alveolar macrophages from horses. Alveolar macrophages were harvested from 6 anesthetized, healthy, light-breed, adult horses by bronchoalveolar lavage. Following a randomized complete block design, the suspension of cells was divided into aliquots of 10/sup 6/ viable alveolar macrophages which were exposed to 1, 10 or 100 g. of opsonized R. equi or opsonized zymosan A at 37 C for 2 hours. In this study the respiratory burst of equine alveolar macrophages was only evidenced by the hexose monophosphate shunt activity and superoxide anion was not coincidentally produced. Rhodococcus equi did not adversely affect that response. The insignificant superoxide anion production by the alveolar macrophages suggests that this may not be a significant oxygen metabolite in those cells.

Research Organization:
Illinois Univ., Urbana (USA)
OSTI ID:
6586467
Country of Publication:
United States
Language:
English

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Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANIMALS
BACTERIAL DISEASES
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CONNECTIVE TISSUE CELLS
DISEASES
ETIOLOGY
FUNCTIONS
GLUCOSE
HEXOSES
HORSES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LAVAGE
LUNGS
MACROPHAGES
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PNEUMONIA
RADICALS
RESPIRATION
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
SACCHARIDES
SOMATIC CELLS
SUPEROXIDE RADICALS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES