Alterations in hematopoietic responses in B6C3F[sub 1] mice caused by drinking a mixture of 25 groundwater contaminants

Journal Article · · Journal of Environmental Pathology, Toxicology and Oncology; (United States)

OSTI ID:6586303

Hong, H L; Yang, R S.H.; Boorman, G A ^[1]

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1. National Institute of Environmental Health Sciences, Research Triangle Park, NC (United States)

Myelotoxicity parameters were monitored in female B6C3F[sub 1] mice exposed to 0, 1, 5, and 10% of a chemical mixture stock in drinking water for 2.5 to 31.5 weeks. The mixture consisted of 25 groundwater contaminants frequently found near toxic waste dumps, as determined by U.S. Environmental Protection Agency (EPA) surveys. Water consumption, body and organ weights, and hematological and histopathological examinations were conducted. No animals developed overt signs of toxicity after 2.5 weeks of treatment. No significant effect on bone marrow cellularity was observed after 2.5, 15.5, or 31.5 weeks of exposure; however, mice exposed to 5% or higher concentrations of the chemical mixture stock solution for 15.5 weeks showed significant suppression of granulocyte-macrophage progenitor cells (CFU-GM) and erythroid precursors (CFU-E) with no changes in body weight, histopathological or hematological parameters. Decreases occurred in erythrocyte mean corpuscular volume of mice exposed to a 10% solution for 15.5 weeks and to 5 and 10% solutions following 31.5 weeks of treatment. In addition, dose-related decreases were found in body, liver, and thymus weights in the 5 and 10% solutions exposure groups after 31.5 weeks. These results suggest that bone marrow may be a sensitive indicator for long-term, low-level exposure of multiple chemicals in mice. Furthermore, long-term exposure to highly contaminated groundwater may present a subtle risk to the hematopoietic system.

• OSTI ID: 6586303
• Journal Information: Journal of Environmental Pathology, Toxicology and Oncology; (United States), Journal Name: Journal of Environmental Pathology, Toxicology and Oncology; (United States) Vol. 11:2; ISSN 0731-8898; ISSN JEPOEC
• Country of Publication: United States
• Language: English

Related Subjects

550900 -- Pathology
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
BODY
BONE MARROW
CFU
CHRONIC EXPOSURE
COLONY FORMATION
CONTAMINATION
DIGESTIVE SYSTEM
DRINKING WATER
GLANDS
GROUND WATER
HAZARDOUS MATERIALS
HEMATOPOIETIC SYSTEM
HYDROGEN COMPOUNDS
LIVER
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MICE
ORGANS
OXYGEN COMPOUNDS
RODENTS
THYMUS
TISSUES
TOXIC MATERIALS
TOXICITY
VERTEBRATES
WATER