Purification and characterization of DNase VII, a 3'. -->. 5'-directed exonuclease from human placenta
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:6583881
An exonuclease, DNase VII, has been purified 6000-fold from human placenta. The enzyme has an apparent molecular weight of 43,000, requires Mg/sup 2 +/ for activity, and has a pH optimum of 7.8. The enzyme hydrolyzes single-stranded and nicked duplex DNA at the same rate proceeding in a 3' ..-->.. 5' direction liberating 5'-mononucleotides. It does not measurably hydrolyze polyribonucleotides.
- Research Organization:
- Johns Hopkins Univ., Baltimore, MD
- OSTI ID:
- 6583881
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 256:15
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
NUCLEASES
STRUCTURAL CHEMICAL ANALYSIS
ENZYME ACTIVITY
ISOTOPE APPLICATIONS
MAN
MOLECULAR WEIGHT
PLACENTA
PURIFICATION
TRITIUM COMPOUNDS
ANIMALS
ENZYMES
ESTERASES
FETAL MEMBRANES
HYDROLASES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
PHOSPHODIESTERASES
PRIMATES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
NUCLEASES
STRUCTURAL CHEMICAL ANALYSIS
ENZYME ACTIVITY
ISOTOPE APPLICATIONS
MAN
MOLECULAR WEIGHT
PLACENTA
PURIFICATION
TRITIUM COMPOUNDS
ANIMALS
ENZYMES
ESTERASES
FETAL MEMBRANES
HYDROLASES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
PHOSPHODIESTERASES
PRIMATES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques