Role of cellular antioxidants (glutathione and ascorbic acid) in the growth and development of wild carrot suspension cultures

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:6582893

Earnshaw, B A

Determinations of endogenous glutathione (GSH), glutathione disulfide (GSSG), ascorbic acid (AA) and dehydroascorbic acid (DHA) in proliferating and developing wild carrot cultures showed that lower levels of GSH and AA were associated with developing cultures. The GSSG and DHA levels did not account for the changes in the levels of antioxidants between proliferating and developing cultures. Studies were designed to test an observed auxin (2,4-Dichlorophenoxyacetic acid, 2,4-D)-antioxidant association. Two fractions (embryo and less developed) were obtained by screening developed cultures which were previously grown in the presence of /sup 14/C-2, 4-D. The embryo fraction had a lower concentration of /sup 14/C than the less developed fraction, supporting the association, since the two fractions showed this relationship with respect to GSH and AA concentrations. Determinations of GSH and AA levels of cells grown in various concentrations of 2,4-D showed the association, decreases in the 2,4-D concentration correlated with decreases in the GSH and AA concentrations. The existence of a respiratory pathway involving GSSG reductase, DHA reductase, and AA oxidase was investigated to test whether inhibition of AA oxidase by 2,4-D could explain the auxin-antioxidant association; however, AA oxidase activity was not detected.

• Research Organization: Institute of Paper Chemistry, Appleton, WI (USA)
• OSTI ID: 6582893
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASCORBIC ACID
BIOLOGICAL FUNCTIONS
GLUTATHIONE
PLANT CELLS
CELL PROLIFERATION
AUXINS
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
CARROTS
CELL CULTURES
ENZYME ACTIVITY
OXIDOREDUCTASES
TRACER TECHNIQUES
DRUGS
ENZYMES
FOOD
FUNCTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PEPTIDES
PLANT GROWTH REGULATORS
PLANTS
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
VEGETABLES
VITAMINS
550201* - Biochemistry- Tracer Techniques