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Effect of morphine on /sup 3/H-thymidine incorporation in the subependyma of the rat: an autoradiographic study

Journal Article · · J. Comp. Neurol.; (United States)
; ;

Following morphine treatment, an autoradiographic study investigated the uptake of /sup 3/H-thymidine by the subependymal cells in the rat brain. /sup 3/H-thymidine was administered subcutaneously to adult, male Sprague-Dawley rats 30 minutes after saline or morphine (19 mg/kg) injection. The animals were sacrified 1 hour after /sup 3/H-thymidine administration. In some experiments the opioid antagonist, naloxone, was given alone 45 minutes before /sup 3/H-thymidine or 125 minutes before morphine treatment. Three areas of the subependyma were evaluated in terms of the percentage labeled cells and number of grains per nucleus, and a dorsal-to-ventral gradiant was described. Morphine treatment significantly increased the number of /sup 3/H-thymidine labeled subependymal cells and number of grains/nucleus within labeled cells. Examination of the distribution of grains/nucleus showed that morphine-treated animals had significantly more cells labeled with 30 or more grains than did saline-injected controls. Prior administration of naloxone blocked the increased /sup 3/H-thymidine uptake in morphine-treated animals but had no significant influence on cell proliferation when administered alone. The data are discussed in terms of morphine's possible dual influence on mechanisms which enhance cell transition from G to S phase and/or which accelerate DNA synthesis once these cells have entered the S phase of cell replication.

Research Organization:
Department of Anatomy, Bowman Gray School of Medicine, Winston-Salem, North Carolina
OSTI ID:
6582859
Journal Information:
J. Comp. Neurol.; (United States), Journal Name: J. Comp. Neurol.; (United States) Vol. 208:2; ISSN JCNEA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
ANALGESICS
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
BRAIN
CELL CONSTITUENTS
CELL NUCLEI
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DNA
DRUGS
HETEROCYCLIC COMPOUNDS
INHIBITION
INJECTION
INTAKE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MORPHINE
NARCOTICS
NERVOUS SYSTEM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
OPIUM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SUBCUTANEOUS INJECTION
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES