Enzymology of acetone-butanol-isopropanol formation: Progress report, February 1, 1988--January 31, 1989
This project will elucidate the molecular properties of solvent-forming enzymes and to understand the mechanisms for regulating the metabolic pathway and the expression of solvent-production genes. Our research has been carried out mostly with two strains of Clostridium beijerinckii (Clostridium butylicum). C. beijerinckii strain B593 is the more versatile one as it produces acetone, n-butanol, ethanol and isopropanol. C. beijerinckii strain B592 produces acetone, n-butanol and ethanol, but not isopropanol, which makes it similar to Clostridium acetobutylicum in product pattern. More recently, we have utilized another solvent-producing organism, Bacillus macerans, in our enzyme studies. B. macerans is a facultative anaerobe which produces high levels of acetone and ethanol under anaerobic conditions, and it also appears to go through a metabolic switch to enter the acetone-producing stage. The metabolic pathway of B. macerans does not have the butyric acid/butanol branches which offers two advantages in our study of solvent formation: (1) it would not have two acetoacetyl CoA-reacting enzymes (3-hydroxybutyryl CoA dehydrogenase and phosphotransbutyrylase) to interfere with the study of acetoacetate-forming enzymes, and (2) it allows a study of the physiology of acetone formation without the complication of accompanying butanol formation. 4 refs., 1 fig., 1 tab.
- Research Organization:
- Virginia Polytechnic Inst. and State Univ., Blacksburg (USA)
- DOE Contract Number:
- FG05-85ER13368
- OSTI ID:
- 6581986
- Report Number(s):
- DOE/ER/13368-5; ON: DE89006290
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Enzymology of acetone-butanol-isopropanol formation
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59 BASIC BIOLOGICAL SCIENCES
ENZYMES
GENE REGULATION
MOLECULAR STRUCTURE
EVALUATION
SOLVENTS
PRODUCTION
ANAEROBIC CONDITIONS
BACTERIA
BIOCONVERSION
MOLECULAR BIOLOGY
PROGRESS REPORT
DOCUMENT TYPES
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