Effects of low dose rate irradiation on plateau phase bone marrow stromal cells in vitro: demonstration of a new form of non-lethal, physiologic damage to support of hematopoietic stem cells
The authors have developed an in vitro system for study of the effects of low dose rate (LDR) irradiation on bone marrow stromal cells. Stromal cultures were irradiated at 5 rad/min, 20 rad/min, or 200 rad/min, 24 hours or 3 weeks prior to engraftment. Two classes of irradiation damage were demonstrated following 1000 rad irradiation at 200 rad/min: 1) Decreased clonagenic survival of trypsinized replated marrow stromal cells (lethal effect); and 2) decreased production by marrow stromal cells or D2XRII cells of colony stimulating factors (CSF)s for granulocyte-macrophage progenitor cells and B6SUtA cells (physiologic effect). Holding the cultures in plateau phase for 3 weeks after irradiation was associated with significantly more repair of the lethal effect compared to the physiologic effect. These data demonstrate a distinct physiologic expression of irradiation damage to bone marrow stromal cells that affects cell to cell interaction, responds differently to changes in dose rate, and is repaired with kinetics different from those of the lethal effect of irradiation. The present system should prove valuable for investigation of cellular interactions in hematopoietic stem cell engraftment that are altered by total body irradiation.
- Research Organization:
- Harvard Medical School, Cambridge, MA
- OSTI ID:
- 6579340
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 10:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BONE MARROW CELLS
BIOLOGICAL RADIATION EFFECTS
COLONY FORMATION
CELL KILLING
RADIOINDUCTION
PROTEINS
RADIOIMMUNOASSAY
BIOLOGICAL REPAIR
IN VITRO
LOW DOSE IRRADIATION
MICE
STEM CELLS
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
IRRADIATION
ISOTOPE APPLICATIONS
MAMMALS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOASSAY
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550301 - Cytology- Tracer Techniques